What is the function of HPRT?
The hypoxanthine-guanine phosphoribosyltransferase (HPRT) assay uses cultured human somatic cells to detect mutagenic agents. The normal function of HPRT in cells is to recycle nucleotide bases from degraded DNA.
What does HPRT stand for?
HPRT
| Acronym | Definition |
|---|---|
| HPRT | Hypoxanthine Phosphoribosyltransferase |
| HPRT | Harvard Program in Refugee Trauma (Massachusetts General Hospital; Cambridge, MA) |
| HPRT | Hydraulic Power Recovery Turbine |
| HPRT | Hypothalamic-Pituitary Radiation Therapy |
Why is HPRT used as a control?
HPRT is a housekeeping enzyme involved in recycling guanine and inosine in the purine salvage pathway. As a housekeeping gene, HPRT has been widely used as an endogenous control for molecular studies evaluating changes in gene expression.
What is HPRT locus?
Study of the hypoxanthine-guanine phosphoribosyl transferase (HPRT; E.C. 2.4. 2.8) locus in cultured animal cells has been intensive for a variety of reasons. HPRT is a purine salvage enzyme which converts preformed purine bases, hypoxanthine and gua- nine, to their respective nucleotides.
Where is the HPRT gene located?
The HPRT gene is located on the long arm of chromosome X (Xq26–q27). In addition, four autosomal nonfunctional HPRT-like sequences, probably pseudogenes, have been identified (two on chromosome 11, one on chromosome 3, and one on chromosome 5). The HPRT gene has been cloned and its sequence determined.
What are the symptoms of Lesch-Nyhan syndrome?
The symptoms of Lesch-Nyhan syndrome include impaired kidney function, acute gouty arthritis, and self-mutilating behaviors such as lip and finger biting and/or head banging. Additional symptoms include involuntary muscle movements, and neurological impairment.
Where is the HPRT1 gene located?
Kidney problems commonly occur in people with this condition because a buildup of uric acid crystals can form kidney stones. Rarely, this condition will cause problems with the nervous system. The HPRT1 gene is found on the X chromosome ( mosome/x/).
How does BestKeeper work?
The BestKeeper algorithm calculates the stability of the candidate genes based on the SD of their Cq values, plus the coefficient of variance (CV), correlation coefficient (r), and the p-value (p) which are also very important parameters (Pfaffl et al., 2004).
How does geNorm work?
geNorm is a popular algorithm to determine the most stable reference (housekeeping) genes from a set of tested candidate reference genes in a given sample panel. From this, a gene expression normalization factor can be calculated for each sample based on the geometric mean of a user-defined number of reference genes.
