What is a Transilluminator used for?
An ultra-violet (UV) transilluminator is a standard piece of equipment used in life science laboratories for visualization of target DNAs and proteins. The UV transilluminator works by emitting high levels of UV radiation through the viewing surface.
How do you use gel doc Transilluminator?
How the Gel Documentation System works. A transilluminator is mounted on roll-out rails inside a light-tight cabinet. To place the gel, the door is opened and transilluminator pulled out. Push it in, close the door and switch on the UV light and camera.
What is UView loading dye?
UView 6x loading dye is a dual-action fluorescent nucleic acid stain and loading dye that allows for immediate visualization of DNA fragments after electrophoresis without the need for in-gel or post electrophoretic staining/destaining.
What is gel documentation system?
Gel documentation systems are used to image labeled nucleic acid and protein samples in various types of media such as agarose, acrylamide or PVDF or nitrocellulose. Gel doc systems come in a variety of configurations depending on throughput and sample type.
What Transillumination means?
Transillumination is the shining of a light through a body area or organ to check for abnormalities.
What makes the DNA move through the gel?
The DNA molecules have a negative charge because of the phosphate groups in their sugar-phosphate backbone, so they start moving through the matrix of the gel towards the positive pole. Power is turned on and DNA fragments migrate through gel (towards the positive electrode).
What is the purpose of gel loading dye?
Purpose. Loading dye is mixed with samples for use in gel electrophoresis. It generally contains a dye to assess how “fast” your gel is running and a reagent to render your samples denser than the running buffer (so that the samples sink in the well).
How much loading dye should you add?
Use 5 µl of Gel Loading Dye, Blue (6X) per 25 µl reaction, or 10 µl per 50 µl reaction. Mix well before loading gel.
Why do you need a UV Transilluminator to observe your DNA bands?
Introduction: UV Transilluminator This technique is used wherever the researcher needs to be able to view their sample, for example sizing a PCR product, purifying DNA segment after a restriction enzyme digest, quantifying DNA or verifying RNA integrity after extraction.
What gel is used in electrophoresis?
The types of gel most typically used are agarose and polyacrylamide gels. Each type of gel is well-suited to different types and sizes of the analyte. Polyacrylamide gels are usually used for proteins and have very high resolving power for small fragments of DNA (5-500 bp).
Does a hydrocele transillumination?
Shining a light through the scrotum (transillumination). If you or your child has a hydrocele, transillumination will show clear fluid surrounding the testicle.
Do epididymal cysts get bigger?
Usually, epididymal cysts and spermatoceles will either get smaller as the body reabsorbs fluid from the cyst or they will stay the same size. Sometimes, though, an epididymal cyst may continue to grow larger or cause pain, swelling, or embarrassment to the patient.
What is the Bio-Rad Education discount?
For more than 15 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows high school and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices. How do I apply for the education discount?
What is gel doc XR+ technology?
The Gel Doc XR+ System is based on CCD high-resolution, high-sensitivity detection technology and modular options to accommodate a wide range of samples and support multiple detection methods including fluorescence and colorimetric detection.
What is the difference between gel doc XR+ and xcitablue?
The XcitaBlue (UV to blue conversion screen) protects samples from UV damage and also allows you to externally visualize DNA samples without a UV shield The Gel Doc XR+ System can be upgraded to the ChemiDoc XRS+ System.
What is the difference between the uview 6x and 6x mini transilluminator?
The UView 6x loading dye is best for DNA electrophoresis applications. The UView transilluminator allows visualization of agarose gels at long wavelengths. The UView 6x mini transilluminator is best for fluorescent applications that require excitation at 365 nm. What is the education discount policy?
