What does a Chemagic do?
Based on our patented chemagen magnetic bead technology the chemagic™ 360 instrument represents the ideal solution for nucleic acid isolation in a huge variety of research market segments including but not limited to Biobanking/Human Genetics, HLA Typing, Virus and Bacteria Detection.
What Chemagic 360?
The chemagic™ 360 instrument: a compact solution for automated DNA and RNA purification. With dimensions of only 80 x 80 cm and its unique sample volume flexibility, the chemagic™ 360 instrument is an innovative system that can be adapted to fit almost all of your automated DNA and RNA purification needs.
How do you elute DNA from magnetic beads?
Eluting DNA Remove the tube containing the pelleted magnetic beads (Step 12, above) from the Magnetic Separator. Add 1 ml Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5) irrespective of the blood volume to the tube and gently agitate the sample by swirling the tube to release the bead pellet from the tube wall.
How does RNA extraction work?
Organic Extraction Methods During centrifugation, the sample separates into three phases: a lower organic phase, a middle phase that contains denatured proteins and gDNA, and an upper aqueous phase that contains RNA. The upper aqueous phase is recovered and RNA is collected by alcohol precipitation and rehydration.
What is QIAsymphony?
The QIAsymphony AS provides active cooling of reagents, eluates, and assays, enabling safe and reproducible assay setup. The QIAsymphony AS enables setup of multiple assays per run or sample and supports QIAGEN PCR and RT-PCR kits. A customization service is available for in-house assays.
How does nucleic acid bind to magnetic beads?
In correct salt concentrations the nucleic acids bind to the magnetic particles. Then a magnet is placed outside of the tube to create a strong external magnetic field. The magnetic particles bound with nucleic acid are attracted to the field and “stick” to the outer edges of the tube.
How do you separate DNA from RNA?
DNA and RNA can also be isolated from the same biological sample by extracting a total nucleic acid fraction and dividing it into two parts – one of which will be treated with a DNase 1 while the other portion will be treated with RNase A to recover RNA and DNA, respectively.
How do you separate RNA?
RNA can be separated from other cellular components by adding chloroform and centrifuging the solution. This separates the solution into two phases: organic and aqueous phases.
