How much do oligos cost?
Single-stranded DNA
| Product | Length | Pricing |
|---|---|---|
| 25 nmole DNA Plate Oligo | 15 – 60 Bases | $0.19 USD / Base |
| 100 nmole DNA Plate Oligo | 10 – 90 Bases | $0.32 USD / Base |
| 250 nmole DNA Plate Oligo | 5 – 100 Bases | $0.54 USD / Base |
| 1 umole DNA Plate Oligo | 5 – 100 Bases | $1.06 USD / Base |
How much does it cost to order primers?
Service Details
| Service Name | Unit | Unit Price |
|---|---|---|
| Primer Design Service | 1 Pair Design | $120.00 |
| Primer Synthesis (25 nmol) | 1 Base | $0.53* |
| Primer Synthesis (100 nmol) | 1 Base | $1.05* |
| Primer Synthesis (250 nmol) | 1 Base | $1.88* |
How are oligos synthesized?
Custom DNA oligos are made by a process called synthesis or more specifically, solid-phase chemical synthesis. This is a method in which the 4 nucleic acids, A, T, C, and G, are added one by one to form a growing chain of nucleotides. They are built on an oligo building block called a phosphoramidite.
What is oligo synthesis scale?
Oligonucleotides are synthetized in amounts (called scales hereafter) of 20 nmol, 40 nmol, 200 nmol, 1 µmol and 10 µmol. The ordered scale is an approximation of the amount of final product.
What is Mer in oligo?
The length of the oligonucleotide is usually denoted by the term “mer,”which is Greek for “part.” Oligonucleotides are used as probes for detecting specific sequences that are complementary to the oligonucleotides.
What is an Ultramer oligo?
Ultramer DNA Oligos are long, single- and double-stranded synthetic DNA sequences. They are manufactured with an optimized synthesis platform that achieves consistent coupling efficiencies over 99.5%. In vitro transcription—templates for the synthesis of RNA.
Is there a hazmat fee on primers?
Shipping Requirements for Hazardous Materials Powder and Primers require a Hazardous Material Handling Fee. $19.50 Hazmat Fee for UPS, $37.50 for Fedex (Signature Require) or $19.50 SpeeDee delivery, you choose carrier at checkout. To keep shipping costs to a minimum, powder and primers may be shipped together.
How do you synthesis a primer?
A primer must be synthesized by an enzyme called primase, which is a type of RNA polymerase, before DNA replication can occur. The synthesis of a primer is necessary because the enzymes that synthesize DNA, which are called DNA polymerases, can only attach new DNA nucleotides to an existing strand of nucleotides.
What is oligo manufacturing?
Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure (sequence). Typically, synthetic oligonucleotides are single-stranded DNA or RNA molecules around 15–25 bases in length.
What is the difference between oligo and primer?
Oligonucleotides are used as probes for detecting specific sequences that are complementary to the oligonucleotides. The primer binds to the specific DNA sequence and DNA polymerase is used to extend the oligonucleotide and replicate the complementary strand.
Are oligonucleotides biologics?
Oligonucleotides are intermediate in size; they are bigger than small molecules, but smaller than biologics, such as peptides and proteins. Oligonucleotide metabolism is mediated by exo- and endo-nucleases.
How many oligonucleotides are there in antisense?
Investigational therapies As of 2020 more than 50 antisense oligonucleotides were in clinical trials, including over 25 in advanced clinical trials (phase II or III).
