How do you reconstitute bmp4?
Reconstitution Recommendation: We recommend that this vial be centrifuged prior to opening. Reconstitute in 5–10 mM Citric Acid, pH 3.0, to a concentration of 0.1–1.0 mg/mL.
Is bmp4 a morphogen?
Bmp-4 acts as a morphogen in dorsoventral mesoderm patterning in Xenopus. Development.
Is BMP4 a regulatory gene?
Genes such as BMP4 and BMP2 are both active within the precursors of the hair shaft. BMP4 is part of the signaling network which controls the development of hair. It is needed for the induction of biochemical pathways and signaling for regulating the differentiation of the hair shaft in the anagen hair follicle.
What is the function of BMP-4?
Like BMP-7, BMP-4 is involved in the development and maintenance of bone and cartilage. Reduced expression of BMP-4 is associated with a number of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva.
What is the molecular weight of recombinant human BMP-4?
PeproTech’s E.coli-derived BMP-4 is a fully active homodimeric protein consisting of two 106 amino acid subunits, which correspond to amino acids 303-408 of the full length BMP-4 precursor. The calculated molecular weight of Recombinant Human BMP-4 (E.coli-derived) is 23.9 kDa.
How long does it take for BMP-4 to work?
12 months from date of receipt, -20 to -70 °C as supplied. Recombinant Human BMP‑4 (Catalog # 314-BP) induces BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED 50 for this effect is 0.70-7.00 ng/mL.
How many amino acids are in BMP 4?
The human BMP-4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfide-linked homodimeric BMP-4 and facilitates its secretion.
