How do you make a thick and thin blood smear?

How do you make a thick and thin blood smear?

Thin smears

1. Place a small drop of blood on the pre-cleaned, labeled slide, near its frosted end.
2. Bring another slide at a 30-45° angle up to the drop, allowing the drop to spread along the contact line of the 2 slides.
3. Quickly push the upper (spreader) slide toward the unfrosted end of the lower slide.

What type of slide would you use for a blood smear?

2. It is easiest to use microscope slides with a frosted end, so that identifying information can be written there with pencil.

How do you stain thick blood film?

Use of Giemsa stain is the recommended and most reliable procedure for staining thick and thin blood films. Giemsa solution is composed of eosin and methylene blue (azure). The eosin component stains the parasite nucleus red, while the methylene blue component stains the cytoplasm blue.

What is the optimum pH range of a buffer used in staining blood smears?

Since distilled water is slightly acid, it was necessary to buffer it to obtain a pH of 7.0 to 7.4, with the optimum pH being 7.2 to 7.4.

What are the causes of too thin or too thick blood smears?

Blood smears that are too thin or too thick present a prob- lem. Extremely thin smears (caused by too small a drop, too slow spreading or too low a spreader angle), may result in red blood cells (RBC) that appear as spherocytes and in – creased white blood cells (WBC), such as monocytes and neutrophils, in the tails.

Why thick smear is not fixed?

Thick smear. It is not fixed in methanol; this allows the red blood cells to be hemolyzed, and leukocytes and any malaria parasites present will be the only detectable elements.

How do you make a good blood smear slide?

1. Place clean glass slide on a flat surface. Add one small drop of blood to one end.
2. Take another clean slide, and holding at an angle of about 45 deg, touch the blood with one end of the slide so the blood runs along the edge of the slide by capillary action.
3. Make 2 smears, allow to air dry, and label clearly.

What is the difference between thin and thick blood film?

A thick blood smear is a drop of blood on a glass slide. A thin blood smear is a drop of blood that is spread across a large area of the slide.

What is the recommended stain for rapid result?

Wright (Wright-Giemsa) stain It can be used if rapid results are needed, but should be followed up when possible with a confirmatory Giemsa stain, so that Schüffner’s dots can be demonstrated.

What is the recommended pH for Wright’s stain?

pH 6.8
The Wright Stain protocol requires a pH 6.8 buffer to produce good quality staining.

Why the spreader slide should be maintained at 30 to 45?

If you go too slow, the smear is too long (lacks a feathered edge). Angle of the spreader slide: The angle determines the length of the smear. An angle of approximately 30-40° is optimal. Maintain this angle through the duration of the spreading action.

Why is thick smear not fixed?

How do you use a blood spreader?

Touch the slide to 1 drop free flowing blood toward one end of the slide. Holding a second slide at 30 degree angle to the first slide, draw back into the drop, and allow it to spread into the edge of the spreader slide. Then quickly and evenly push the spreader slide forward, allowing the blood to spread out.

What should I do if my spreader settings are incorrect?

Improper spreader settings may result in uneven fertilization, turf injury, inadequate pest control, and a waste of time and money. When using a LESCO product, find the designated setting listed on the bag and then cross reference that number to your particular spreader type using the charts below.

How do you prepare thick blood film for a blood test?

Prepare thick blood film by applying a free-flowing drop of blood, preferably without an anticoagulant, to a microscope slide cleaned with alcohol to remove any oil present. Using the corner of a second slide, spread the film in a circle about the size of a dime.

How do you make a blood film buffer for histology?

Prepare the working buffer by adding 61.1 ml stock buffer 1 and 38.9 ml stock buffer 2 to 900 ml distilled water. Prepare thick blood film by applying a free-flowing drop of blood, preferably without an anticoagulant, to a microscope slide cleaned with alcohol to remove any oil present.