How do you make a spectinomycin stock solution?
Weigh 0.5-1 g of spectinomycin [Spectinomycin /Dihydrochloride Pentahydrate GoldBio Catalog # S- 140, (CAS 21736-83-4, MW = 495.3 g/mol)]. 2. Add 10 ml of sterile H2O. Dissolve completely.
Does chloramphenicol dissolve in water?
Solubility : Soluble in water (2.5 g/l) at 25° C, methanol, ethanol, butanol, ethyl acetate, acetone, DMSO, and propylene glycol (150.8 mg/ml).
What is the mechanism of action of spectinomycin?
Spectinomycin is an aminocyclitol aminoglycoside antibiotic derived from Streptomyces spectabilis with bacteriostatic activity. Spectinomycin binds to the bacterial 30S ribosomal subunit. As a result, this agent interferes with the initiation of protein synthesis and with proper protein elongation.
What class of antibiotic is spectinomycin?
It is in the aminocyclitol class of drugs and works by stopping the making of protein by certain bacteria. Spectinomycin was discovered in 1961.
How do you make Rifampinin stock solution?
Weigh 0.5 g of rifampicin [Rifampicin, GoldBio Catalog # R-120, (CAS 13292-46-1, MW = 822.94 g/mol)]. 2. Add 10 ml of 100% methanol. Dissolve completely.
How do you make tetracycline stock solution?
Preparation of 80ml stock solution
- Tetracycline is kept in the 4C fridge in 68-564D.
- Weigh 400mg of tetracyline HCL into a small weigh boat.
- Dilute 95% Ethanol to 70% using milliQ water.
- Add 80ml of 70% Ethanol to a 250ml bottle.
- Add the tetracycline HCL to the ethanol.
What is the best solution to dilute chloramphenicol?
Intravenously as a 10% (100 mg/mL) solution to be injected over at least a one-minute interval. This is prepared by the addition of 10 mL of an aqueous diluent such as water for injection or 5% dextrose injection. Adults should receive 50 mg/kg/day in divided doses at 6-hour intervals.
What is spectinomycin used to treat?
Trobicin (spectinomycin) For Injectable Suspension is an antibiotic used to treat gonorrhea. Common side effects of Trobicin include nausea, dizziness, soreness at the injection site, hives, insomnia, chills, and fever.
How does spectinomycin differ from the other aminoglycosides antibiotics?
Spectinomycin is an aminocyclitol antibiotic, which shares similar features with an aminoglycoside. However, it differs in that it does not contain amino sugars or glycosidic bonds. It has a broad spectrum of activity. It is highly water soluble and is easily mixed in aqueous solutions.
Is spectinomycin the same as streptomycin?
Spectinomycin can also be substituted for streptomycin in some cases, and provides more stability, but when increased stability is not required, streptomycin should be used since it is much more cost effective.
How to prepare spectinomycin stock solution?
Volume of spectinomycin stock: Spectinomycin concentration: Step 1. Prepare a spectinomycin stock solution ( ) by adding. spectinomycin dihydrochloride. Step 2. Dissolve in dH 2 O and filter-sterilize. Step 3. Make aliquots and store at -20°C.
What is spectinomycin used for in cell culture?
Spectinomycin also has use in amplification of low copy number plasmid carrying replicons. It is recommended for use in cell culture applications at 7.5-20 mg/L. Mode of Action: Spectinomycin acts by inhibiting protein synthesis and elongation by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation.
What is the best way to store spectinomycin?
Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria. Solutions can be stored at 2-8°C for several weeks or at -20°C for more extended periods. Stock solutions should be prepared at 10 mg/mL in water and 0.2 μm filtered. Keep container tightly closed in a dry and well-ventilated place.
What is spectinomycin dihydrate used for?
Spectinomycin also has use in amplification of low copy number plasmid carrying replicons. It is recommended for use in cell culture applications at 7.5-20 mg/L. Spectinomycin dihydrochloride pentahydrate has been used as a supplement in different types of media.
